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International Journal of Basic Science and Technology

A publication of the Faculty of Science, Federal University Otuoke, Bayelsa State

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Archive | ISSUE: , Volume: Oct-Dec-2024

The Cytotoxic and Endocrine Disrupting Potential of Alcohol Ethoxy Sulfates in Oreochromis niloticus Adrenocortical Steroidogenic Cells Exposed In Vitro


Author:Ariyo, A.B. and Ikpesu, T.O.

published date:2024-May-24

FULL TEXT in - | page 232 -239

Abstract

The cytotoxic and endocrine-disrupting potential of surfactant alcohol ethoxy sulfates in a common cichlid Oreochromis niloticus was investigated. The concentration ranges of AES in the field (1.00, 1.50, 2.00, and 2.50) mg/L, including the control, were exposed to the fish for 30 days. Glutathione-s-transferase (GST) and Reduced Glutathione (GSH) and cortisol levels were measured using a UV–VIS Spectrophotometer. There was no significant difference (p > 0.05) in the physiochemical parameters between the experimental group and the control. The effects of the surfactant on GST activity on day 2 of the exposure was slightly affected but was not significant (P>0.5), while days 9 to 16 witnessed moderate alteration that was significant at (P< 0.05) at the concentration of 2.0 and 2.5mg/L only. The changes were obvious on days 23rd and 30th in all the treatments and were highly significant (p<0.01). GSH activity in the blood serum of the investigated fish exposed to different concentrations of AES was not significantly (p > 0.05) affected. However, the enzyme activity was slightly higher than the control in all the treatments. In the control fish, there was a gradual increase in the cortisol level from day 2 (5.20) ug/dl to day 30 (5.30 + 0.010) ug/dl. In the treated fish, the increment in the cortisol level was proportional to the exposure concentration and was significant (p < 0.05) between the control and various treatments. Detergents seep into groundwater and enter rivers, harming fish and, indirectly, humans. By choosing the safest hazardous products, consumers can reduce chemical pollution

Keywords: Alcohol ethoxy sulfates, Endocrine-disrupting Oreochromis niloticus Surfactant,,,

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