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×published date:2015-Oct-30
FULL TEXT in - | page 39-42
Abstract
Sea-foods are regarded as reservoirs for potentially pathogenic Vibrio species. This study investigated the prevalence of medically important Vibrio species from sea-foods obtained from markets in Lagos. A total of 174 samples made up of 58 crabs, 44 shrimps and 35 fishes were collected from major markets in Lagos metropolis. Isolation was done on Thiosulphate Citrate Bile Salt Sucrose (TCBS) agar. Suspected colonies from the TCBS agar were subjected to Gram’s reaction, oxidase test, motility test, triple sugar iron, lactose fermentation and salt tolerance tests. Toxigenic capabilities of isolated species were determined using the rabbit ileal loop assay. Antibiotics susceptibility pattern of all the isolates was investigated. Out of the 174 samples, 137 (78.74%) were positive for Vibrio species, all the crabs were contaminated with Vibrios, while 44 (58%) of the shrimps and 35 (87.5%) of the fishes were reservoirs of Vibrio spp. Vibrio parahaemolyticus was the most predominant specie with 45 (32.85%) of the total isolated species, this was followed by Vibrio alginolyticus 28 (20.44%). The result of the rabbit ileal loop assay of the isolated strains showed that all the V.mimicus strains possessed toxigenic ability, while 39.29% of V.alginolyticus and 31.58% of Vibrio cholerae exhibited fluid inducible capabilities. Antibiotics sensitivity pattern revealed total susceptibility of strains to ofloxacin, pefloxacin and ciprofloxacin, while strains showed varying susceptibilities to amoxicillin, augmentine, cotrimoxazole and tetracycline. Proper cooking of sea-foods before consumption could reduce the risk factor of exposure to Vibrio species infections.
Keywords: Sea-foods, Vibrio species, Vibrio parahaemolyticus,,
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