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International Journal of Basic Science and Technology

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Archive | ISSUE: , Volume: Jan-Mar-2024

Antibiotics Susceptibility Pattern of Pseudomonas aeruginosa isolated from Obafemi Awolowo University Ile Ife Oxidation Pond


Author:Oladeji et al., 2024

published date:2024-Apr-26

FULL TEXT in - | page 81 - 90

Abstract

Oxidation ponds offer a cost-effective, natural method for treating municipal wastewater, preventing environmental hazards associated with direct disposal into the environment. This study aimed to identify, characterize, and determine the antibiotic susceptibility pattern of Pseudomonas aeruginosa isolates found in Obafemi Awolowo University (OAU) oxidation pond. Five (5) wastewater samples were aseptically obtained from the OAU for physicochemical and microbiological analyses. The samples were processed using standard bacteriological methods. The samples collected for DO and BOD were also analyzed using Winkler’s titration method. Sixteen (16) isolates of the total forty-two (42) isolates were confirmed as P. aeruginosa. The mean microbial loads ranged from 2.1 x 104 to 1.15 x 107 CFU/ml respectively. As regards each pH, temperature, and total dissolved solids (TDS), the null hypothesis was not rejected as the p-values were greater than 0.05. P-values for pH, temperature, and TDS were 0.990, 0.996 and 0.984 respectively. This implies that there was no significant difference in the mean values of each physicochemical parameter obtained during each sampling period of this study. The dissolved oxygen was zero for all samples and the BOD value was between 1.2±0.00 mg/L and 6.4±0.00 mg/L. 87.5% of the P. aeruginosa were resistant to Imipenem, 100% to Amoxicillin-Clavunate and 94% to both Cefuroxime and Ampiclox. None of the isolates were resistant to Gentamicin, Ofloxacin, and Levofloxacin (69%, 100%, and 100% susceptibility respectively). P. aeruginosa thrives in anoxic conditions, making it unlikely to be extensively or pan-drug resistant. However, Gentamicin and Ofloxacin can be effective treatments for community-acquired infections

Keywords: Wastewater, Antibiotic Resistance, Pseudomonas aeruginosa, Physiochemical Parameter,

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