Sub acute toxicity studies of Phyllanthus amarus on haematological parameters and some plasma enzymes activities in mice

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Abstract

Abstract: Haematological parameters and plasma enzymesactivities were investigated in mice treated with sub-lethal concentration of ethanol extractof Phyllanthusamarus. Twenty healthy male mice weighing between 28- 31g were randomly divided into four groups (A- D) of five rats each.Group A were orally dosedwith 1ml of distilled water, while the other groups (B - D) were administered with 1000, 1500, 2000mg/kg body weight of the extract, after seven days treatment period, the mice were anaesthetised and blood samples collected via cardiac puncture for analysis. All doses of theextract under investigation did not significantly (P > 0.05) alter the level of Hb, PCV, RBC, MCH, MCHC, MCV and platelet count, but however induced a significant decrease (P< 0.05) in the level of white blood cell,a significance increase (P< 0.05) was also observed at the dose of 1500 and 2000mg/kg body weight in AST, ALT, ALP and LDH levels, an indication that sub-acute oral doses of P. amarusmay compromise immune response and induce organ damage.

Keywords: Haematology,mice, Phyllanthus amarus,plasma enzyme, toxicity.

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