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×published date:2025-Apr-16
FULL TEXT in - | page 418 - 429
Abstract
This study aimed to evaluate the hepatoprotective effect of I. cairica leaf extract against the hepatotoxic effect of diclofenac in male Wistar rats. Thirty-five male Wistar rats were randomly divided into five groups of seven rats each. I: normal control; II: orally administered 100 mg/kg of diclofenac (DCF); III: orally administered 100 mg/kg Methanolic extract of I. cairica (MEIC) for 14 days before single dose administration of DCF; IV: orally administered 250 mg/kg of MEIC for 14 consecutive days before single dose administration of DCF; V: orally administered 250 mg/kg of MEIC for 14 consecutive days. Animals were sacrificed 24 hours after the last administration. Blood was collected and processed for markers of lipidaemia and hepatic function, the liver was excised and processed for antioxidant (glutathione [GSH], catalase [CAT], superoxide dismutase [SOD], oxidative stress (malondialdehyde [MDA] and protein carbonyl [PC], and pro-inflammatory activities (xanthine oxidase (XO), myeloperoxidase (MPO), and NADP(H) oxidase).The results from the experiment confirmed the pro-oxidative effect of DCF as observed in the increased concentration of MDA and PC, decreased activities of the antioxidant enzymes, and increased activities of the pro-inflammatory enzymes as compared to the control (P<0.05). It also showed that DCF induced an increase in AST activity (P<0.05) in the serum. Pretreatment with MEIC was able to prevent the oxidative and inflammatory effect of DCF in the liver. Conclusion: The results provide a scientific proof of the potential of I. cairica to improve the ability of liver to counter the toxic effect of DCF overdose.
Keywords: Hepatotoxicity, Inflammation, Natural products, Oxidative stress, Diclofenac
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