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International Journal of Basic Science and Technology

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Archive | ISSUE: , Volume: Oct-Dec-2023

Bacterial Profile of Asymptomatic Bacteriuria and High Prevalence of TetA Resistant Genes among Students in a Tertiary Institution in Nigeria.


Author:Olajide et al.,2023

published date:2024-May-12

FULL TEXT in - | page 220 - 231

Abstract

Asymptomatic bacteriuria (ASBU) is a condition in which urine culture reveals a significant growth of bacteria that is greater than 105 bacteria/ml, but without the patient showing symptoms of urinary tract infection (UTI). In about 70% of the cases, ASBU is a major risk factor for the occurrence of UTI in an individual. This cross-sectional investigation was carried out among 160 healthy students of LAUTECH, Ogbomosho, Nigeria. Data containing information that predisposes the students to the risk of UTI and urine samples were collected. Bacteria were isolated using standard microbiological techniques, antibiotic susceptibility to 11 antibiotics and detection of tetracycline-resistant genes tetA, tetB and tetC using the disc diffusion and PCR methods respectively was done. Bacteria were isolated from 18 (11.3%) samples while 142 (88.7%) samples had no significant growth. Escherichia coli and Klebsiella species (3.7%) were the most frequently isolated followed by Staphylococcus aureus (2.5%) and Pseudomonas species (1.3%). The isolates were more susceptible to Gentamicin and Imipenem and were mostly resistant to Tetracycline. Molecular characterization of tetracycline resistance revealed that 5 E. coli (83.3%), and 6 Klebsiella spp. (100%) isolates resistance was due to the tetA gene only, 50% of Pseudomonas aeruginosa isolates contained the tetB gene while none of the bacteria carried the tetC gene. The prevalence of tetracycline resistance is a setback hindering its use in the treatment of infection and a limit to its adoption as a treatment option in combination therapy. Students should be educated on healthy toilet habits and indiscriminate use of antibiotics.

Keywords: Asymptomatic bacteriuria, UTI, Students, Escherichia coli Klebsiella species,

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