In Vitro Morphogenetic Response of Moringa oleifera Lam. Leaf Petiole Explant to Cytokinin and Auxin Concentrations

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Abstract

The study investigated the morphogenetic response that can be obtained from M. oleifera leaf petiole explant in vitro. Murashige and Skoog (MS) medium was supplemented with different concentrations and combinations of plant growth regulators. The concentrations (1.0, 2.0, 3.0, 4.0, 5.0, 6.0, 7.0, 8.0 and 9.0 mg/l) of Indole acetic acid (IAA) and 2,4-Dichlorophenoxyacetic acid (2,4-D) were used singly and in combination with 0.1 mg/l Benzyl amino purine (BAP) for media supplementation. The results obtained seven days after culture initiation indicated that different morphogenetic responses can be obtained from M. oleifera leaf petiole culture. When plant growth regulators were used singly for media supplementation, IAA favored callus and root formation, while 2,4-D supported callus and shoot development, Cultures containing combinations of both IAA and 2,4-D hormones resulted in the formation of calli, roots and shoots.. A plantlet was obtained from the culture supplemented with 7.0 mg/l IAA and 0.1 mg/l BAP after 30 days of culture initiation.

Keywords: callus, growth regulators, in vitro, leaf petiole, Moringa oleifera

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