ISSN : 2488-8648
International Journal of Basic Science and Technology
A publication of the Faculty of Science, Federal University Otuoke, Bayelsa State
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ISSN : 2488-8648
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×published date:2015-Oct-30
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Abstract
Plant sample preservation is critical in maintaining the integrity of tissues prior to DNA extraction, especially in cases where the tissue collection site is distanced from the laboratory. This study attempts to investigate methods of preserving oil palm (Elaeis guineensis Jacq.) leaf tissue prior to DNA extraction. Oil palm was preserved using physical method by storing leaf samples in the freezer (-20°C), fridge (4°C), room temperature (25°C) and sun drying (37°C). Furthermore, some samples were preserved using chemical method by soaking them in 95% Ethanol, Salted ethanol, SDS buffer and 1X TAE buffer. Both methods were for duration of 2, 4, 6 and 8 days, prior to nucleic acid extraction. Both DNA yield and DNA purity were determined with Spectrophotometric analysis. Also, the purity of the DNA extract from tissues preserved using physical method was confirmed with Agarose gel electrophoresis. When compared with nucleic acid extracts from unpreserved fresh plant tissue (control), the results obtained indicated that preservation at -20°C and storage in Salted ethanol were most effective for long term storage of oil palm leaf samples. Preservation in TAE buffer seemed to be the least effective preservation method as there was low DNA yield obtained from the tissues stored in it. The purity of DNA extracts was not affected by both the physical and chemical preservation methods under investigation.
Keywords: Preservation, Elaeis guineensis, DNA extraction, Spectrophotometric analysis, Gel electrop
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