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International Journal of Basic Science and Technology

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Archive | ISSUE: , Volume: Oct-Dec-2015

In Vitro Morphogenetic Responses of Dioscoreophyllum cumminsii (Stapf) Diels Seed Explant


Author:Isikhuemen O. Y., Nwaoguala C. N. C., Odewale J.

published date:2015-Oct-20

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Abstract

This study was undertaken to investigate the in vitro morphogenetic responses of Dioscoreophyllum cumminsii (Stapf) Diels (serendipity berry) using seed explant. A combination of different plant growth regulators (PGRs) were used; five concentrations of Naphthalene acetic acid (NAA) and five concentrations of 6-Benzyl amino purine (BAP) or Kinetin, to supplement Murashige and Skoog (MS) medium. The highest callus intensity was observed in the media containing 25mg/l NAA + 1mg/l kinetin and also in the media containing 5mg/l NAA + 2mg/l BAP. With regard to organogenesis, it was observed that 2.5mg/l NAA alone produced the highest number of rootlets and 2mg/l BAP alone produced taller plantlets. The results obtained in the study indicated that morphogenetic responses of D. cumminsii could be by NAA in combination with BAP or Kinetin, to undergo callogenesis, shoot formation or/and rhizogenesis based on the ratio of the growth regulators.

Keywords: Auxin, Callogenesis, Cytokinin, Dioscoreophyllum cumminsii , Rhizogenesis

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