ISSN : 2488-8648
Home About IJBST For Authors Issues Useful Downloads Contact
Questions are asked and these questions need answers. This is the reason why this page is created to enable us share few worries!
×published date:2015-Oct-20
FULL TEXT in - | page 1-6
Abstract
This study was undertaken to investigate the in vitro morphogenetic responses of Dioscoreophyllum cumminsii (Stapf) Diels (serendipity berry) using seed explant. A combination of different plant growth regulators (PGRs) were used; five concentrations of Naphthalene acetic acid (NAA) and five concentrations of 6-Benzyl amino purine (BAP) or Kinetin, to supplement Murashige and Skoog (MS) medium. The highest callus intensity was observed in the media containing 25mg/l NAA + 1mg/l kinetin and also in the media containing 5mg/l NAA + 2mg/l BAP. With regard to organogenesis, it was observed that 2.5mg/l NAA alone produced the highest number of rootlets and 2mg/l BAP alone produced taller plantlets. The results obtained in the study indicated that morphogenetic responses of D. cumminsii could be by NAA in combination with BAP or Kinetin, to undergo callogenesis, shoot formation or/and rhizogenesis based on the ratio of the growth regulators.
Keywords: Auxin, Callogenesis, Cytokinin, Dioscoreophyllum cumminsii , Rhizogenesis
Abiodun, O.A. and Akinoso, R. (2014). Physico-chemical properties of serendipity berry (Dioscoreophyllum cumminsii) fruit. Journal of Applied Science and Environmental Management, 18 (2) 218-221.
Adansi, M.A. and Holloway, H.L.O. (1977). Seed germination and establishment of the serendipity berry, Dioscoreophyllum cumminsii. Diels. Acta Horticulturae, 53:407-411.
Ammirato, P.V. (2004).Yams.In: Ammirato, P.V., Erans, D.A., Sharp, W.R. and Yamada, Y. (eds). Handbook of Plant cell culture, Vol. 3. Macmillan, New York, pp. 329-354.
Ata, J.K.B.A. and Hammonds, T.W. (1976). A note on oil extracted from Serendipity berry (Dioscoreophyllum cumminsii. Diels), Ghana Journal of Agricultural Science, 9:63-64.
Gaba, V.P. (2005). Plant growth regulators in plant tissue culture. In: Trigiano, R.N. and Gray, D.J (eds). Plant development and biotechnology. CRC press, Boca Raton, London, New York, Washington D.C. 348p.
Holloway, H.L.O. (1977). Seed propagation of Dioscoreophyllum cumminsii, source of an intense sweetener. Economic Botany. 31:17-50.
Hussain, A., Qarshi, I.A., Nazir, H. and Ullah, I. (2012). Plant tissue culture: Current status and opportunities. In: Leva A. andRinaldi L.M.R. (eds) Recent advances in plant in vitro culture. In Tech, Rijeka, p. 1-28
IITA. (2008). Yam research for development, IITA publication. 1:1-10.
Inglett, G.E. (1981). Unusual sweeteners of plant origin.Food technology, 35 (3) 31-41.
Murashige, T. and Skoog, F. (1962).A realized medium for rapid growth and bioassays with tobacco cultures.Plant Physiology, 15: 473 – 497.
Obioh, G.I.B and Isichei, A.O. (2007).A population viability analysis of serendipity berry (Dioscoreophyllum cumminsii) in a semi-deciduous forest in Nigeria. Ecological modeling. 201:558-562.
Okoro, O.O. (1976). Propagation of Dioscoreophyllum cumminsii. Nigerian Journal of Forestry, 6:70.
Okoro, O.O. (1980). Propagation of Dioscoreophyllum cumminsii. Nigerian Journal of Forestry, 10:48-57.
Oselebe, H.O., Ene-Obong, E.E. and Nwankiti, O.C. (2004). The biology of Dioscoreophyllum cumminsii (Stapf) Diels. Nigerian Journal of Botany, 17:17-28.
Oselebe, H.O and Ene-Obong, E.E. (2007). Organogenesis in Dioscoreophyllum cumminsii (Stapf) Diels. Tropicultura, 25(1) 37-43.
Seyyed R. S.,Behzad K. and Naghi P.D. (2013). The effect of different concentrations of NAA and BAP on micropropagation of Alstroemeria. European Journal of Experimental Biology, 3(5):133-136.
Skoog, F. and Miller, C.O. (1957). Chemical regulation of growth and organ formation in plant tissue cultured In-vitro. Symp. Soc. Exper. Biol. 11:118-131.
FULL TEXT in - | page 1-6
Issue 4-Oct-Dec
Issue 3-Jul-Sep
Issue 2-Apr-Jun
Issue 1-Jan-Mar
Issue 4-Oct-Dec
Issue 3-Jul-Sep
Issue 2-Apr-Jun
Issue 1-Jan-Mar
Issue 4-Oct-Dec
Issue 3-Jul-Sep
Issue 2-Apr-Jun
Issue 1-Jan-Mar
Issue 4-Oct-Dec
Issue 2-Apr-Jun
Issue 1-Jan-Mar
Issue 4-Oct-Dec
Issue 3-Jul-Sep
Issue 4-Oct-Dec
Issue 2-Apr-Jun
Issue 1-Jan-Mar
Issue 4-Oct-Dec
Issue 3-Jul-Sep
Issue 2-Apr-Jun
Issue 4-Oct-Dec
Issue 1-Jan-Mar
Copyright © International Journal of Basic Science and Technology | Faculty of Science, Federal University Otuoke 2019. All Rights Reserved.
P.M.B. 126, Yenagoa. Bayelsa state Nigeria
Get the most recent updates
and be updated your self...