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Archive | ISSUE: , Volume: Jan-Mar-2023

Investigation of the Biochemical Indices Related to Differential Response of Fasting Blood Glucose to Alloxan Induced Diabetic Rats


Author:Martin, O., Blessing, O. O. and Gloria, O. O

published date:2023-Apr-03

FULL TEXT in - | page 26 - 33

Abstract

Alloxan-induced diabetes has been noted to provoke the generation of free radicals with eventual damage to the β-cells of the pancreas. Alloxan or its resultant effects on the pancreas could affect the functionality of other organs and systems, hence the reason for the assessment of some organ functions and numerous biochemical indices for the prognosis of diabetes. The unpredictability of alloxan has also been widely reported. Apart from the reports of blood glucose that is mostly used as a prognostic index of diabetes mellitus, the influence of unstable alloxan-induced diabetes on organ functions and many other prognostic indices are either not researched or scanty. This study examined the variability of biochemical indices in unstable alloxan-induced diabetes. Alloxan (150 mg/kg) was administered to 20 male Wistar rats while 5 male Wistar rats were used as control. Rats injected with alloxan were further shared into 2 groups: diabetic control and reversed diabetic (rats with normal fasting blood glucose post-diabetic confirmation). The rats were observed for 2 weeks and were allowed access to water and food as much as they wanted. Antioxidant enzymes, liver and kidney functions, and hematological indices were evaluated after 2 weeks while blood glucose was analyzed weekly. The results showed inconsistent alteration in some of the indices (Fasting blood glucose, mean corpuscular hemoglobin concentration (MCHC), alanin aminotransferase (ALT), and Na+ by both diabetes and reversed diabetes groups in relation with the control. Most of the parameters were unchanged in all the groups. These findings support our earlier report that biochemical alteration by alloxan may not depend only on the glucose level.

Keywords: Alloxan, Diabetes, β-cells, Hematological, Reversed diabetes

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